CircGAK inhibits cell growth, migration, invasion, and angiogenesis of hepatocellular carcinoma via miR-1323/HHIP axis

Abstract Increasing evidence demonstrates that circular RNA (circRNA) plays a pivotal role in the development of disease, especially Cancer. A previous circRNA microarray study showed circGAK (hsa_circ_0005830) was remarkably down-regulated hepatocellular carcinoma (HCC) tissues. However, HCC remains largely unclear. The candidate circRNAs were screened via integrating Gene Expression Omnibus (GEO) database (GSE164803) analysis with online program GEO2R. Quantitative real-time PCR (qRT-PCR) employed to measure expression miR-1323, and hedgehog-interacting protein (HHIP) tissues cells. biological function examined using colony formation assay, 5-ethynyl-2'-deoxyuridine (EdU) wound healing transwell cell invasion endothelial tubular western blot xenograft mouse model. Bioinformatics analysis, immunoprecipitation (RIP) dual-luciferase reporter assay utilized test interaction between or HHIP. abnormally associated tumor-node-metastasis (TNM) stage. Overexpression impeded proliferation, migration, invasion, tube vitro, tumor growth vivo. predicted interacted which targeted HHIP mRNA 3'untranslated regions (UTR). Furthermore, upregulation miR-1323 shRNA-mediated suppression could recover circGAK-mediated malignant behaviors cells Taken together, circGAK/miR-1323/HHIP axis suppress progression may provide potential new targets for diagnosis therapy HCC.